MYCOsiRNA:
an effective and specific siRNA designer for fungi and oomycete RNAi based on the assessments of Target site accessibility and Genome-wide Off-target selection
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Please paste your sequence in FASTA format:
[Load demo data]
-Only accept one sequence every time
-Uplimit of submission: 15k
siRNA length
siRNA GC% between
Target accessibility
Maximum # of off-target genes
Database for off-target analysis
nt
to
%
Select a species
Magnaporthe oryzae
Botrytis cinerea
Puccinia spp.
Fusarium graminearum
Fusarium oxysporum
Blumeria graminis
Mycosphaerella graminicola
Colletotrichum spp.
Ustilago maydis
Melampsora lini
Phytophthora infestans
Hyaloperonospora arabidopsidis
Phytophthora ramorum
Phytophthora sojae
Phytophthora capsici
Plasmopara viticola
Phytophthora cinnamomi
Phytophthora parasitica
Pythium ultimum
Albugo candida
Select motifs to avoid in the designed siRNAs
'AAAA' in antisense strand
'UUUU' in antisense strand
'CCCC' in antisense strand
'GGGG' in antisense strand
'UGGC' in antisense strand
>2×'CUG' in antisense strand
>2×'CCG' in antisense strand
>2×'CGG' in antisense strand
